In vitro regeneration of pakistani peanut

Reference Micropropagation of Prosopis cineraria L. The influence of environment, media and Zertol on forcing and in vitro establishment of softwood shoots from large segments of Acer saccharinum L. Propagation of Ornamental Plants 5 3

In vitro regeneration of pakistani peanut

NS NS One of the remarkable feats of earlier physiological analysis on in vitro organogenesis was the identification of the predominant role of auxin and cytokinin as chemical determinants in plant development. It has been reported that these cytokinins promoted shoot regeneration from explants of most of the grain legumes[9].

The effect of BA on shoot bud induction was attributed to the ability of plant tissues to metabolize the natural hormone more readily than other, synthetic, growth regulators or to induce endogenous production of zeatin.

It is an efficient cytokinin and is commonly used in plant tissue culture. Definite and significant pattern of response was obtained in all the genotypes. Maximum number of buds was produced in the MS media fortified with Hence, these genotypes responded equally in terms of inducing caulogenic buds with reference to BA Fig.

Unpredictably, one or two caulogenic bud like induction was noted in explants cultured in MS media without PGR control. Callus was not observed at this stage of culture and all caulogenic buds seemed to develop de novo i.


Callusing was observed in control. It is therefore evident in this study that BA is capable of inducing caulogenic buds in these groundnut varieties and the genotypes responded similarly.

Our result suggests that protocol followed for caulogenic bud induction in SB 11 can be applied to TAG 24 and WesternHB and similar results can be expected. Hence, the cultivars like TAG 24 and Western can be applied as a model system for gene transfer techniques.

Caulogenic bud induction frequency in different concentrations of BA among genotypes TDZ is a potent cytokinin that promotes shoot formation and plant regeneration via organogenesis and somatic embryogenesis in cultivated peanut within a short duration while in culture[2,11,12].

Later studies confirmed the effective use of TDZ as the sole growth-regulating compound for the induction of regeneration in many species like Phaseolus vulgaris[14], Peanut[15,16], Cicer[17] etc.

TAG 24 showed high caulogenic response in Callus was observed at this stage of culture in some explants but all caulogenic buds seemed to develop de novo i. Control explants showed callus significantly. In this study, the concentrations of TDZ used here were the equivalent concentrations to that of BA so that a potential comparable data can be obtained.

It is interesting to note that WesternHB responded well in the lower concentration of TDZ in comparison to SB 11 and TAG 24, thus making it very effective in producing more caulogenic buds as well as their proper growth and development of plantlets.

Microscopic observation of double-stained normal and morphogenic mass caulogenic buds showed that the cells from morphogenic masses were very large compared to normal cells Fig. They had large nuclei and stained bright red by acetocarmine compared to control. The cytoplasm and vacuole of some cells are also stained by acetocarmine, confirming the caulogenic status of the cells.

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Histological sections of cells obtained by double staining of cells in control and morphogenic mass obtained in TDZ 4. Indirect caulogenesis in TDZ was observed to some extent however direct caulogenesis was mostly observed. The morphogenic nature of the cells was confirmed with histological staining methods.

When comparing the effects of TDZ and BA with respect to caulogenic response, it can be inferred from these values that TDZ is a more potent inducer of caulogenesis in vitro than BA by producing more buds.

All three genotypes responded equally to different concentrations of BA. Its maximum response at lower concentrations of TDZ 4. Among the Spanish varieties only SB 11 has been exploited for transformation studies.

The explants of WesternHB produced caulogenic buds through direct organogenesis pathway compared to other germplasm. These explants can be targeted through biolistic gun delivery for desired protein or vaccines expression in their caulogenic buds. More the production of caulogenic buds proportional to protein expression not even requiring regeneration procedures.

With regeneration protocol, WesternHB germplasm can be used for nuclear transformation studies. Also, it can become an effective target for breeding approaches in peanut improvement. Planta, In Vitro Cell Develop Biol. Plant, 35, Plant, 34,proved to be the best responsive variety for in vitro regeneration in terms of number of shoots/explant (%) and number of rooted plants/explant (%).

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The optimized protocol, which is the. vitro reproductive behavior of five genotypes of A. paraguariensis. Day-lengths of 12, 16 and 24 h were tested to Day-lengths of 12, 16 and 24 h were tested to monitor in vitro flowering using growth chambers kept at 26˚C ± 1˚C and 60% ± 5% relative humidity under an illumi-.

In peanut, there have been several decades of research on development of plant regeneration systems based on organogenesis (Eapen and George ; Matant et al.

In vitro regeneration of pakistani peanut

In vitro multiplication of banana plantlets is an excellent alternate and a number of countries in the world like Israel (Israeli et al., ), France (Cote et al., ), Australia (Drew & Smith ), Cuba and many African countries (Vuylsteke, ) are.

Peanut (Arachis hypogaea L.) belongs to the family leguminosae and is one of the world’s largest oilseed study is the first report on peanut regeneration from Pakistan using four commercially released peanut varieties, that is, Golden, BARI, BARD and BARD However, in this laboratory, an efficient and improved protocol for in vitro plant regeneration (with a survival rate of >75%) and genetic transformation from cotyledonary node explants of peanut (efficiency 42% approx.) has been developed and being used for .

Crop Science Abstract - In Vitro Plant Regenation of Peanut From Seed Explants | Digital Library